Dna molecular weight marker ii, diglabeled sigmaaldrich. New england biolabs sells a wide range of doublestranded dna molecular weight markers for electrophoresis. Restriction digests pcr rtpcr use this marker to size dna fragments between 0. Perfect dna 1 kbp ladder novagen 70537 emd millipore. We also offer markers suitable for use in pulsefield gel electrophoresis pfge extending up to kb. Dna molecular weight marker vi is a fragment mixture prepared by cleavage of pbr328 dna with restriction endonucleases bgl i and pbr328 dna digested with hinf i. Fragments containing the 12 base cossites of lambda may anneal upon storage. We offer conventional dna markers as well as a wide range of dna ladders, ranging from 10 bp up to 48. It simplifies accurate molecular weight determination of doublestranded dna fragments generated by.
A molecular weight marker is a molecule used to provide an estimate of the size of molecules subjected to gel electrophoresis. Dna molecular weight marker ii, diglabeled is a solution in 10 mm trishcl, 1 mm edta, ph 8. Perfect dna 1 kbp ladder novagen evenly spaced dna markers in readyto load format find msds or sds, a coa, data sheets and more information. The product is also available as dna molecular weight marker vi, digoxigeninlabeled. This is a technique in which dna, rna, or proteins are separated by size, using an electrical current on a gel. Molecular weight markers invitrogen seeblue plus2 prestained protein standard allows you to easily visualize protein molecular weight ranges during electrophoresis and quickly evaluate western transfer efficiency. Dna molecular weight marker iv is used for the size determination of dna in agarose gels. The report molecular weight marker market by products dna, rna. Genomic dna qc using standard gel electrophoresis for. This term should not be confused with molecular markers in genetics areas in which an organisms dna differs from the rest of the population and can be. The product is also available as dna molecular weight marker iii, digoxigeninlabeled. This leads to a gel pattern where one band is of lower intensity than expected or absent completely and a larger fragment has an increased intensity. Invitrogen 100 bp dna ladder is designed for sizing and approximate quantification of doublestranded dna in the range of 100 bp to 2,000 bp.
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